Chiapas (Mexico) and California share many similar challenges regarding the conservation of rivers and streams and the organisms that dwell in them. Although fishes in both states are highly diverse, this biodiversity is under multiple threats including effects from cattle grazing, forest fires, dam operations, and climate change. Collaboration between the El Ocote Biosphere Reserve and the Klamath National Forest began in 1993 in order to provide research expertise and training between the two forests. Past efforts have documented fish species presence and distribution within the Reserve (2005-2008; Gonzalez-Diaz et al. 2008, Anzueto-Calvo et al. 2013), as well as identified threats to aquatic habitats (2010-2012).